Composite

Part:BBa_K4759218:Design

Designed by: Jianhua Chen   Group: iGEM23_Jiangnan-China   (2023-10-07)


T7-RBS1-RBM3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1426
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 472
    Illegal BsaI.rc site found at 1396
    Illegal SapI.rc site found at 952
    Illegal SapI.rc site found at 1552


Design Notes

(BM3 Redox domain)：a gene coding for Reducatase domain from P450BM3(the selfsufficient P450 enzyme). it has an excellent electron transfer system and the fastest substrate oxidation rate among the P450s owing to the proximity and specific positioning of its domains . Researcher has found that fusing P450s to reductase genes can improve the biocatalysis, We use this part to construct electron transfer pathway to Olep.

Source

(BM3 Redox domain)：a gene coding for Reducatase domain from P450BM3(the selfsufficient P450 enzyme).

References